Listeria monocytogenes challenge test

Changes as from 1 January 2022

The new challenge test procedure for Listeria monocytogenes will apply as from 1 January 2022. But what does the new European Technical Guidance Document (TGD) mean for the Food Business Operator (FBO) in practice? And what must you do to comply with these new guidelines? Hence the focus on three changes.

Change 1: Product knowledge

From now on, the FBO must provide the laboratory with sufficient product information for the challenge test. This is necessary in order to adequately take into account all factors that may have an effect. Therefore, you must prepare the challenge test with sufficient product knowledge, respecting the variability between different batches and the historical data from previous tests.

Change 2: Measuring moments

In the past, challenge tests for Listeria monocytogenes growth were done by analysing three batches in triplicate at the beginning and end. As from 1 January 2022, the three batches will still be mandatory, but the measuring times and multiples have changed.

Change 3: Storage temperature

In order to predict listeria growth in food, Europe is now quoting 10°C at the consumer stage. It used to be 12°C. But in Belgium, the temperature remains at 9°C. So if you produce for the Belgian market, that lower temperature takes precedence over the European requirement.


Of course, the story is much more nuanced than those three general outlines. The presentation goes into great detail so that you can fully understand the new regulations.

Register for the Dutch-language online training at 10 a.m. on Thursday, 6 January

Our experts will give you the most important points for attention in 45 minutes. So be sure to reserve a suitable time:

After we have received your details, you will receive an email with the URL to log in to the online presentation on 6 January.